Collaborator Login
 Methods
Cellular Response Measurements v 1.0. The goal of the Cellular Response Laboratory (CRL) is to define leukocyte number and function in patients following treatment with hematopoietic stem cell transplantation.
Video
Multiparameter Flow Cytometric High Content Screening (FC-HCS). 
7-Amino-Actinomycin D Staining Of Dead Cells For Flow Cytometry v 1.0. Staining protocol for live/dead discrimination of cells. 7-Amino-actinomycin D (7-AAD) intercalates into double-stranded nucleic acids. It is excluded by viable cells but can penetrate cell membranes of dying or dead cells.
Video
Bone Marrow Thawing Protocol v 1.0. 
Video
FDG Staining v 1.0. Staining mammalian cells with FDG, a fluorescent substrate for ß-galactosidase
Video
Long Term Bone Marrow Culture Media (Gartner's) v 1.0. 
Video
Monocyte (MØ) Depletions with Plastic Adherence v 1.0. 
Video
Mononuclear Cell Separation v 1.0. 
Video
Preparation of Retroviral Vector Supernatants v 1.1. 
Video
Retroviral Infection Of Cell Suspension V 1.0. 
Video
Procecure For Thawing And Dextran Wash Of Cryopreserved Cells v 1.0. 
Video
Screening Knockout And Transgenic Mice For Hematopoietic Phenotypes. Phenotypic screening of transgenic and knockout mice for disorders in hematopoietic cell self renewal, differentiation and function will provide useful insights into the genetic basis of hematopoietic biology and diseases.
Video
BAAA Staining Protocol v2.0. 
Video