Collaborator Login
 Projects
Ex-vivo expansion of hematopoietic stem cells

Defining the role of E2F transcription factors in HSC self renewal and differentiation.
We are using a combination of molecular and cell biologic techniques including retroviral vector gene transfer to explore the role of the E2F transcription factors in mediating HSC self renewal and differentiation. see more...
This project is belongs to the Nevins Lab collaboration space. Login required for complete access.

Ex-vivo expansion of human hematopoietic stem cells (HSCs).
We have developed an automated high throughput flow cytometric technique termed Flow Cytometric High Content Screening (FC-HCS) that we are using to analyze “combinatorial libraries” of culture conditions in order to identify those that enhance HSC expansion. see more...
This project is belongs to the Smith Lab collaboration space.

Screening knockout and transgenic mice for genes that effect HSC self renewal and differentiation.
We are using the Flow Cytometric High Content Screening technique to systematically analyze the stem cell and progenitor compartments in knockout and transgenic mice. see more...
This project is belongs to the Smith Lab collaboration space.
Flow Informatics

Bioinformatics Collaborative Grant Application
We are planning to apply for a grant to support data exchange and analysis standards for high throughput flow cytometry. see more...
This project is belongs to the Flow Informatics collaboration space.

Java for Flow Cytometry
We are developing a collection of Java packages that should be useful for reading list mode data and applying gates and regions. see more...
This project is belongs to the Flow Informatics collaboration space.

Statistical Software for Analysis of Flow Data Using R
Functions and collections of functions (packages) for statistical analysis of FACS data including gating. see more...
This project is belongs to the Flow Informatics collaboration space.

XML Standards for Data and Analysis Exchange
We are developing XML standards for the exchange of data and for the description of analyses including gates and regions. see more...
This project is belongs to the Flow Informatics collaboration space.
Identification of novel hematopoietic stem cells

Identification and characterization of novel human hematopoietic stem cells.
A variety of clinical and laboratory evidence now indicates that hematopoietic stem cells which do not express CD34 may be important stem cell populations. see more...
This project is belongs to the Smith Lab collaboration space.

Identification, characterization and transplantation of hematopoietic stem cells with high levels of ALDH expression.
We have developed a fluorescent substrate, termed BAAA, for the enzyme Aldehyde Dehydrogenase (ALDH) that allows the enumeration and isolation of primitive hematopoietic cells. see more...
This project is belongs to the Smith Lab collaboration space.
Immune reconstitution following stem cell transplant

Mapping cellular immune reconstitution following stem cell transplantation.
We have established a laboratory called the Cellular Response Lab (CRL) that uses the FC-HCS technology to define immune recovery following transplantation of hematopoietic stem cells. see more...
This project is belongs to the Smith Lab collaboration space.